pcad antibody Search Results


anti cdh3 antibody  (Proteintech)
93
Proteintech anti cdh3 antibody
Direct transcriptional activation of <t>CDH3</t> by KLF4 in HCC cells. (A) The two potential binding sites for KLF4 in the promoter of CDH3. (B) CDH3 promoter reporters (pGL3-CDH3-627 and pGL3-CDH3-439) were transfected into 239T cells in triplicate with KLF4 expression plasmids or control vectors for 24 h. The CDH3 promoter activity was then examined using a dual luciferase assay kit. (C) The CDH3 promoter reporter activities were determined in KLF4 overexpressed Huh7 and Hep3B cells. (D) The CDH3 promoter reporter activities were determined in KLF4 silenced YY-8103 and HCC-LM3 cells. The experiments were performed independently three times. *P<0.05, **P<0.01. (E) Chromatin immunoprecipitation assays were performed with a specific anti-KLF4 antibody or IgG and oligonucleotides flanking the KLF4 binding sites were amplified by PCR. ( F ) The activities of mutant CDH3 promoters were determined by dual luciferase assays in KLF4 overexpressed Huh7 cells.
Anti Cdh3 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cdh3 antibody/product/Proteintech
Average 93 stars, based on 1 article reviews
anti cdh3 antibody - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
biotin conjugated secondary antibody  (Proteintech)
86
Proteintech biotin conjugated secondary antibody
Direct transcriptional activation of <t>CDH3</t> by KLF4 in HCC cells. (A) The two potential binding sites for KLF4 in the promoter of CDH3. (B) CDH3 promoter reporters (pGL3-CDH3-627 and pGL3-CDH3-439) were transfected into 239T cells in triplicate with KLF4 expression plasmids or control vectors for 24 h. The CDH3 promoter activity was then examined using a dual luciferase assay kit. (C) The CDH3 promoter reporter activities were determined in KLF4 overexpressed Huh7 and Hep3B cells. (D) The CDH3 promoter reporter activities were determined in KLF4 silenced YY-8103 and HCC-LM3 cells. The experiments were performed independently three times. *P<0.05, **P<0.01. (E) Chromatin immunoprecipitation assays were performed with a specific anti-KLF4 antibody or IgG and oligonucleotides flanking the KLF4 binding sites were amplified by PCR. ( F ) The activities of mutant CDH3 promoters were determined by dual luciferase assays in KLF4 overexpressed Huh7 cells.
Biotin Conjugated Secondary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin conjugated secondary antibody/product/Proteintech
Average 86 stars, based on 1 article reviews
biotin conjugated secondary antibody - by Bioz Stars, 2026-04
86/100 stars
  Buy from Supplier
rat anti-pcad antibody  (Becton Dickinson)
90
Becton Dickinson rat anti-pcad antibody
Direct transcriptional activation of <t>CDH3</t> by KLF4 in HCC cells. (A) The two potential binding sites for KLF4 in the promoter of CDH3. (B) CDH3 promoter reporters (pGL3-CDH3-627 and pGL3-CDH3-439) were transfected into 239T cells in triplicate with KLF4 expression plasmids or control vectors for 24 h. The CDH3 promoter activity was then examined using a dual luciferase assay kit. (C) The CDH3 promoter reporter activities were determined in KLF4 overexpressed Huh7 and Hep3B cells. (D) The CDH3 promoter reporter activities were determined in KLF4 silenced YY-8103 and HCC-LM3 cells. The experiments were performed independently three times. *P<0.05, **P<0.01. (E) Chromatin immunoprecipitation assays were performed with a specific anti-KLF4 antibody or IgG and oligonucleotides flanking the KLF4 binding sites were amplified by PCR. ( F ) The activities of mutant CDH3 promoters were determined by dual luciferase assays in KLF4 overexpressed Huh7 cells.
Rat Anti Pcad Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti-pcad antibody/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
rat anti-pcad antibody - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Direct transcriptional activation of CDH3 by KLF4 in HCC cells. (A) The two potential binding sites for KLF4 in the promoter of CDH3. (B) CDH3 promoter reporters (pGL3-CDH3-627 and pGL3-CDH3-439) were transfected into 239T cells in triplicate with KLF4 expression plasmids or control vectors for 24 h. The CDH3 promoter activity was then examined using a dual luciferase assay kit. (C) The CDH3 promoter reporter activities were determined in KLF4 overexpressed Huh7 and Hep3B cells. (D) The CDH3 promoter reporter activities were determined in KLF4 silenced YY-8103 and HCC-LM3 cells. The experiments were performed independently three times. *P<0.05, **P<0.01. (E) Chromatin immunoprecipitation assays were performed with a specific anti-KLF4 antibody or IgG and oligonucleotides flanking the KLF4 binding sites were amplified by PCR. ( F ) The activities of mutant CDH3 promoters were determined by dual luciferase assays in KLF4 overexpressed Huh7 cells.

Journal: International Journal of Biological Sciences

Article Title: KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling

doi: 10.7150/ijbs.30857

Figure Lengend Snippet: Direct transcriptional activation of CDH3 by KLF4 in HCC cells. (A) The two potential binding sites for KLF4 in the promoter of CDH3. (B) CDH3 promoter reporters (pGL3-CDH3-627 and pGL3-CDH3-439) were transfected into 239T cells in triplicate with KLF4 expression plasmids or control vectors for 24 h. The CDH3 promoter activity was then examined using a dual luciferase assay kit. (C) The CDH3 promoter reporter activities were determined in KLF4 overexpressed Huh7 and Hep3B cells. (D) The CDH3 promoter reporter activities were determined in KLF4 silenced YY-8103 and HCC-LM3 cells. The experiments were performed independently three times. *P<0.05, **P<0.01. (E) Chromatin immunoprecipitation assays were performed with a specific anti-KLF4 antibody or IgG and oligonucleotides flanking the KLF4 binding sites were amplified by PCR. ( F ) The activities of mutant CDH3 promoters were determined by dual luciferase assays in KLF4 overexpressed Huh7 cells.

Article Snippet: Immunohistochemical analysis was performed with anti-KLF4 (1:200, Proteintech, 11880-1-AP) and anti-CDH3 antibody (1:50, Proteintech, 13773-1-AP).

Techniques: Activation Assay, Binding Assay, Transfection, Expressing, Control, Activity Assay, Luciferase, Chromatin Immunoprecipitation, Amplification, Mutagenesis

CDH3 expression is activated by KLF4 in HCC cells. (A) KLF4 overexpression by transient transfection promoted CDH3 expression by qRT-PCR. (B) The mRNA level of CDH3 detected by qRT-PCR was reduced in KLF4 stably silenced HCC cells. *P<0.05, **P<0.01. (C) Overexpression of KFL4 enhanced CDH3 expression at protein level in WRL68 and YY-8103 cells detected by western blot analyses. (D) Western blots showing the expression pattern of KLF4 and CDH3 in a series of HCC cell lines.

Journal: International Journal of Biological Sciences

Article Title: KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling

doi: 10.7150/ijbs.30857

Figure Lengend Snippet: CDH3 expression is activated by KLF4 in HCC cells. (A) KLF4 overexpression by transient transfection promoted CDH3 expression by qRT-PCR. (B) The mRNA level of CDH3 detected by qRT-PCR was reduced in KLF4 stably silenced HCC cells. *P<0.05, **P<0.01. (C) Overexpression of KFL4 enhanced CDH3 expression at protein level in WRL68 and YY-8103 cells detected by western blot analyses. (D) Western blots showing the expression pattern of KLF4 and CDH3 in a series of HCC cell lines.

Article Snippet: Immunohistochemical analysis was performed with anti-KLF4 (1:200, Proteintech, 11880-1-AP) and anti-CDH3 antibody (1:50, Proteintech, 13773-1-AP).

Techniques: Expressing, Over Expression, Transfection, Quantitative RT-PCR, Stable Transfection, Western Blot

CDH3 expression is associated with KLF4 level in HCC specimens. (A) TMA specimens including 64 HCC tissues were prepared for immunostaining with specific antibodies against CDH3 and KLF4. Representative images of CDH3 and KLF4 expression in HCC specimens were shown. (B) Data showed that the CDH3 expression levels directly correlated with the KLF4 expression levels in HCC. (C) A positive correlation between KLF4 and CDH3 mRNA level in additional 37 paired HCC tissues was measured by linear regression (r=0.675, P<0.001).

Journal: International Journal of Biological Sciences

Article Title: KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling

doi: 10.7150/ijbs.30857

Figure Lengend Snippet: CDH3 expression is associated with KLF4 level in HCC specimens. (A) TMA specimens including 64 HCC tissues were prepared for immunostaining with specific antibodies against CDH3 and KLF4. Representative images of CDH3 and KLF4 expression in HCC specimens were shown. (B) Data showed that the CDH3 expression levels directly correlated with the KLF4 expression levels in HCC. (C) A positive correlation between KLF4 and CDH3 mRNA level in additional 37 paired HCC tissues was measured by linear regression (r=0.675, P<0.001).

Article Snippet: Immunohistochemical analysis was performed with anti-KLF4 (1:200, Proteintech, 11880-1-AP) and anti-CDH3 antibody (1:50, Proteintech, 13773-1-AP).

Techniques: Expressing, Immunostaining

Downregulation of CDH3 induced cell proliferation and migration. (A) Colony formation assays were performed to detect the growth of LV-shCDH3-infected Focus, HCC-LM3 and WRL68 cells and control cells, respectively. Colonies were counted and captured. The levels of CDH3 expression were examined via western blot. *P<0.05. (B) Transwell assays were performed to detect changes in migratory abilities of Focus, HCC-LM3 and WRL68 cells treated with LV-shCDH3 or LV-shNC control. (C) KLF4 expression plasmid was transfected into CDH3 silenced HCC cells and control cells, respectively. Transwell chamber assays were then performed to detect cell migration of these cells.

Journal: International Journal of Biological Sciences

Article Title: KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling

doi: 10.7150/ijbs.30857

Figure Lengend Snippet: Downregulation of CDH3 induced cell proliferation and migration. (A) Colony formation assays were performed to detect the growth of LV-shCDH3-infected Focus, HCC-LM3 and WRL68 cells and control cells, respectively. Colonies were counted and captured. The levels of CDH3 expression were examined via western blot. *P<0.05. (B) Transwell assays were performed to detect changes in migratory abilities of Focus, HCC-LM3 and WRL68 cells treated with LV-shCDH3 or LV-shNC control. (C) KLF4 expression plasmid was transfected into CDH3 silenced HCC cells and control cells, respectively. Transwell chamber assays were then performed to detect cell migration of these cells.

Article Snippet: Immunohistochemical analysis was performed with anti-KLF4 (1:200, Proteintech, 11880-1-AP) and anti-CDH3 antibody (1:50, Proteintech, 13773-1-AP).

Techniques: Migration, Infection, Control, Expressing, Western Blot, Plasmid Preparation, Transfection

The effect of CDH3 overexpression on growth and migration of HCC cells. (A) Cell proliferation of Huh7, YY-8103 and Sk-Hep-1 cells transfected with CDH3 plasmid or control empty vector was evaluated using CCK8 assay. And the level of CDH3 expression was examined using western blot. *P<0.05. (B) Transwell assays were conducted to evaluate migration of CDH3 overexpressed Huh7, YY-8103 and Sk-Hep-1 cells.

Journal: International Journal of Biological Sciences

Article Title: KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling

doi: 10.7150/ijbs.30857

Figure Lengend Snippet: The effect of CDH3 overexpression on growth and migration of HCC cells. (A) Cell proliferation of Huh7, YY-8103 and Sk-Hep-1 cells transfected with CDH3 plasmid or control empty vector was evaluated using CCK8 assay. And the level of CDH3 expression was examined using western blot. *P<0.05. (B) Transwell assays were conducted to evaluate migration of CDH3 overexpressed Huh7, YY-8103 and Sk-Hep-1 cells.

Article Snippet: Immunohistochemical analysis was performed with anti-KLF4 (1:200, Proteintech, 11880-1-AP) and anti-CDH3 antibody (1:50, Proteintech, 13773-1-AP).

Techniques: Over Expression, Migration, Transfection, Plasmid Preparation, Control, CCK-8 Assay, Expressing, Western Blot

CDH3 affects GSK-3β expression in HCC cells. (A) Western blot analysis was used to measure the expressions of GSK-3β, p-GSK-3β, AKT, p-AKT, Erk, p-Erk, P70S6K, p-P70S6K, SMAD3, p-SMAD and β-catenin in CDH3 silenced Focus cells. (B) The levels of GSK-3β and p-GSK-3β were examined in CDH3 overexpressed YY8103 and HCC-LM3 cells using western blot assay. The cells were treated with or without insulin for 10 min. (C) TCF/LEF luciferase activity was measured by dual luciferase reporter kit and the results illustrated that CDH3 expression had no effect on the transcription activity of Wnt/β-catenin signaling. (D) The GSK-3β knockdown cell line was constructed by transfecting siGSK-3β into Focus and YY-8103 cells and detected by western blotting. (E) The migration assay was conducted in Focus and YY-8103 cells after transfection with siGSK-3β (or control).

Journal: International Journal of Biological Sciences

Article Title: KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling

doi: 10.7150/ijbs.30857

Figure Lengend Snippet: CDH3 affects GSK-3β expression in HCC cells. (A) Western blot analysis was used to measure the expressions of GSK-3β, p-GSK-3β, AKT, p-AKT, Erk, p-Erk, P70S6K, p-P70S6K, SMAD3, p-SMAD and β-catenin in CDH3 silenced Focus cells. (B) The levels of GSK-3β and p-GSK-3β were examined in CDH3 overexpressed YY8103 and HCC-LM3 cells using western blot assay. The cells were treated with or without insulin for 10 min. (C) TCF/LEF luciferase activity was measured by dual luciferase reporter kit and the results illustrated that CDH3 expression had no effect on the transcription activity of Wnt/β-catenin signaling. (D) The GSK-3β knockdown cell line was constructed by transfecting siGSK-3β into Focus and YY-8103 cells and detected by western blotting. (E) The migration assay was conducted in Focus and YY-8103 cells after transfection with siGSK-3β (or control).

Article Snippet: Immunohistochemical analysis was performed with anti-KLF4 (1:200, Proteintech, 11880-1-AP) and anti-CDH3 antibody (1:50, Proteintech, 13773-1-AP).

Techniques: Expressing, Western Blot, Luciferase, Activity Assay, Knockdown, Construct, Migration, Transfection, Control